|Year : 2017 | Volume
| Issue : 3 | Page : 147-151
Quantitative changes in anaerobic subgingival microbiota in patients, before and during fixed orthodontic treatment
Amit Sidana1, Ragni Tandon2, Shrish C Srivastava3
1 Senior Lecturer, Maharaja Gangasingh Dental College, Sri Ganganagar, Rajasthan, India
2 Prof. and HOD, Department of Orthodontics and Dentofacial Orthopedics, Saraswati Dental College, Lucknow, Uttar Pradesh, India
3 Reader, Department of Orthodontics and Dentofacial Orthopedics, Saraswati Dental College, Lucknow, Uttar Pradesh, India
|Date of Submission||07-Oct-2016|
|Date of Acceptance||04-Apr-2017|
|Date of Web Publication||17-Jul-2017|
Department of Orthodontics and Dentofacial Orthopedics, Saraswati Dental College, Tiwari Ganj, Lucknow - 226 016, Uttar Pradesh
Source of Support: None, Conflict of Interest: None
Aim: The aim of this study was to compare the growth of Actinobacillus actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, and Veillonella before and during fixed orthodontic treatment. Materials and Methods: The study sample consisted of 40 patients (20 males, 20 females). The patients were divided into two groups, Group 1 (pretreatment group) before any fixed orthodontic treatment and Group 2 (active treatment group) 6 months into fixed treatment. A. actinomycetemcomitans was cultured on tryptic soy bacitracin vancomycin agar, and for the rest and total aerobic count, Columbia agar was used. The culture plates were incubated anaerobically for 72 h. Bacteria were identified by their physical and microscopic appearances. Further, specific bacteria were identified by VITEK 2 Compact Automated Identification System (Biomerieux). Using the magnifying glass, the total number of bacteria was determined. Result: For all the microbes, P. gingivalis, P. intermedia, Veillonella (excepting A. actinomycetemcomitans), a significant increase in count was observed. Mean change was highest for P. intermedia (0.64 ± 0.74 × 104 CFU/ml) and minimum for P. gingivalis (0.12 ± 0.35 × 104 CFU/ml). For A. actinomycetemcomitans, at both before and active treatment phases, the count was 0 ± 0 × 104 CFU/ml. For different microbes, change in microbial count ranged from 18.8% (P. gingivalis) to 52% (P. intermedia). For Veillonella, the change was 51.4%. Conclusion: Orthodontic appliance serves as different loci for bacterial growth. In this in vivo study, significant differences were noted between the bacterial count in pretreatment group and active treatment group. Adequate oral prophylaxis instructions should be given to patients before starting fixed orthodontic treatment so that oral hygiene can be maintained.
Keywords: Actinobacillus actinomycetemcomitans, fixed orthodontics, subgingival fluid
|How to cite this article:|
Sidana A, Tandon R, Srivastava SC. Quantitative changes in anaerobic subgingival microbiota in patients, before and during fixed orthodontic treatment. J Indian Orthod Soc 2017;51:147-51
|How to cite this URL:|
Sidana A, Tandon R, Srivastava SC. Quantitative changes in anaerobic subgingival microbiota in patients, before and during fixed orthodontic treatment. J Indian Orthod Soc [serial online] 2017 [cited 2017 Oct 18];51:147-51. Available from: http://www.jios.in/text.asp?2017/51/3/147/210905
| Introduction|| |
Orthodontic treatment is associated with some amount of deterioration in periodontal health due to plaque accumulation. This compromised periodontal health status occurs due to increase in pathologic bacteria.,, Orthodontic treatment makes the process of maintaining oral hygiene more difficult and more time consuming. The bonded appliances have many undercuts which act as sites for food lodgments and plaque colonization. The overall ecology of the oral cavity is modified greatly by increase in these pathologic bacteria., Poor oral hygiene has resulted in enamel decalcification and gingival inflammation. Gingivitis and periodontitis are associated with pathologic microflora. Actinobacillus is an age-related microorganism which is present in the oral cavity.,,, It is highly pathologic and is responsible for causing localized juvenile periodontitis.,Tannerella forsythia, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, and Prevotella intermedia/nigrescens have also been found in patients with inflammation and periodontal pockets as well as in healthy individuals. Literature has shown that there are very limited studies which determine the rise of Actinobacillus in patients wearing fixed orthodontic appliance., Similarly, there are many other anaerobic pathologic bacteria which also increase during fixed orthodontic treatment which have not been investigated in literature. Studies on other pathogens such as Prevotella and P. gingivalis have been scantly reported.,, To fill this lacuna, the purpose of this study was to quantitatively evaluate the increase in these specific pathogens during orthodontic treatment.
| Materials and Methods|| |
Written consent forms were taken from each of the patients after being informed about the nature of the study in detail. The study was approved by the Local Ethical Committee of Saraswati Dental College and Hospital, Lucknow. The sample consisted of 40 patients (20 males and 20 females) who reported to Department of Orthodontics and Dentofacial Orthopaedics, Saraswati Dental College, Lucknow, for treatment.
- Inclusion criteria:
- DMFT index should be zero
- Good oral hygiene
- No deleterious habits such as smoking and tobacco chewing
- No oral and systematic diseases or syndromes.
- Exclusion criteria:
- Gingivitis, periodontitis, or even mild inflammation
- Antibiotic intake in the last 6 months
- Pregnancy, systemic illness, and fluoride use.
The individuals were divided into two groups: Group A (pretreatment group) before any fixed orthodontic treatment and Group B (active treatment group) 6 months of active fixed orthodontic treatment. The samples were numbered 1–40 with respective to Groups A and B. Subgingival fluid samples were taken from the proximal site of the banded molar before and after band placement. Sterile paper point was inserted to the bottom of the periodontal sulcus and kept in place for 20 s and then put in sterile saline. Tryptic soy serum bacitracin vancomycin agar is an enriched selective medium for the isolation and presumptive identification of A. actinomycetemcomitans. TSBV medium contains bacitracin and vancomycin at a concentration that inhibits most Gram-positive and most Gram-negative anaerobes, except for A. actinomycetemcomitans among others. TSBV agar was prepared by adding 3.25 g of tryptic soy agar in 100 ml distilled water. The culture medium was sterilized by autoclaving for 15 min at 121°C under 15 lbs pressure. This hot media was poured in sterilized petri plates under sterilized laminar air flow. After 15 min, the liquid was cooled to room temperature, and 1.2 ml of stock solution of bacitracin and 1 ml of 50 mg/mL stock solution of vancomycin were added and mixed by gently shaking the container. Preformed agar plate of Columbia blood agar was used for total anaerobic culture. The subgingival fluid sample was vortexed for 15–20 s. Adequate amount of subgingival fluid sample was evenly spread onto both culture mediums. Both culture plates were incubated anaerobically in anaerobic jars using gas packs for 72 h. The appearance of small, translucent, and slightly convex circular colonies with a star-like inner structure was seen in case of A. actinomycetemcomitans in the TSBV medium [Figure 1]. Using the magnifying glass, the total number of the colonies of Actinobacillus was calculated. After 3 days, culture plates were examined for evaluating the total anaerobic count [Figure 2] and specific count of three anaerobic bacteria (P. gingivalis, P. intermedia, and Veillonella).
Specific bacteria were identified by their physical and microscopic appearance. Further, bacteria were identified by VITEK 2 Compact Automated Identification System (Biomerieux) [Figure 3]. The VITEK 2 is an automated microbiology system utilizing growth-based technology. Individual substrates had been measured based on various metabolic activities such as acidification, alkalinization, enzyme hydrolysis, and growth in the presence of inhibitory substances. There are reagent cards available for the identification of different organism classes as follows: GN - Gram-negative fermenting and nonfermenting bacilli, GP - Gram-positive cocci and nonspore-forming bacilli, ANC - Anaerobic card [Figure 3], YST - yeasts and yeast-like organisms, and BCL - Gram-positive spore-forming bacilli. Method of VITEK 2 system was as follows, from anaerobic cultured plate colony was abstracted and subcultured again on anaerobic blood agar. After 3 days, pure culture of specific bacteria was procured. 1–3 colonies were abstracted, and a uniform suspension was prepared in ID tube. Suitable card was placed in respective tubes in barcoded cassettes. At the end, the cassette was loaded in VITEK 2 Compact system which incubates and interprets the results automatically.
The statistical analysis was done using Statistical Package for Social Sciences version 15.0 statistical analysis software (SPSS Inc., IBM, Armonk, New York, US). The values were represented in number (%) and mean ± standard deviation (SD). All variances are reported as SDs difference among means were analyzed by the paired t-test.
| Results|| |
Pretreatment baseline microbial count for P. intermedia was 1.25 ± 1.04 × 104 CFU/ml, for Veillonella was 0.70 ± 0.61 × 104 CFU/ml, and for P. gingivalis was 0.64 ± 0.64 × 104 CFU/ml. A.actinomycetemcomitans was the least common microbe with a mean count of 0.00 ± 0.00 × 104 CFU/ml [Table 1]. Mean anaerobic count was 2.69 ± 1.27 × 105 CFU/ml. In active treatment, microbial count for P. intermedia was 1.89 ± 1.10 × 104 CFU/ml, for Veillonella was 1.06 ± 0.63 × 104 CFU/ml, and for P. gingivalis was 0.76 ± 0.72 × 104 CFU/ml. A. actinomycetemcomitans was the least common microbe with a mean count of 0.00 ± 0.00 × 104 CFU/ml [Table 2]. Total anaerobe count was 2.93 ± 1.33 × 105 CFU/ml. For different microbes, change in microbial count is explained in [Table 3] and [Graph 1 [Additional file 1]]. For anaerobes, the change was higher both for mean count as well as proportional change [Table 4] and [Graph 2 [Additional file 2]].
|Table 1: Baseline microbial count in salivary specimen of patients enrolled in the study (n=40)|
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|Table 2: Microbial count in salivary specimen of patients enrolled in the study during active treatment (n=40)|
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|Table 3: Comparison of change in count of different microbes from baseline to active phase of treatment|
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| Discussion|| |
During active treatment, the important microbial count for the different bacteria had increased except for A. comitans. Same results were seen in a previous study done by Haffajee and Socransky. They reported that important periodontal microorganisms such as P. gingivalis, P. intermedia, and Fusobacterium species were higher after bracket placement. A. actinomycetemcomitans was detected in 7.5% of the total sample in the active treatment group. One of the major reasons for low level of A. actinomycetemcomitans was the presence of increased level of P. gingivalis. A. actinomycetemcomitans and P. gingivalis produce mutual antagonistic products. This result was also seen in the study done by Naranjo et al. They found statistically insignificant findings regarding A. actinomycetemcomitans. Paolantonio et al. did a cross-sectional study on 34 individuals; in which, 20 individuals (test group) underwent orthodontic treatment with fixed appliances for at least 6 months. 85% of test individuals and 47.5% of test sites were positive for A. actinomycetemcomitans in comparison to 28.5% of control individuals and 3.5% of control sites. Paolantonio et al. did longitudinal study and obtained the same result as in cross-sectional study. A. actinomycetemcomitans as a possible pathogen in localized juvenile periodontitis and P. gingivalis were suggested to be important in adult periodontitis. Asikainen et al. isolated the A. actinomycetemcomitans from 2% of sites from periodontally healthy teenagers.
In this study, we compared the subgingival microbiota composition in patients who were scheduled for orthodontic treatment before and after placement of brackets. After 6 months, the change in proportion of anaerobic bacteria is statistically significant. These changes could be observed within 3 months after placement of appliances, which is in accordance with other reports discuss the effect of orthodontic treatment on the periodontium., The diversity in subgingival microbiota described using a microbial cultural technique in patients having orthodontic treatment. Other method to this study particularly found important microorganism that is related to inflammation and periodontal diseases grouped into complex according to their colonization capacities and virulence.
In patients undergoing orthodontic treatment, their periodontal condition should be supervised carefully. Removable and fixed orthodontic appliances block correct oral hygiene resulting in more plaque accumulation, inflammation, and bleeding. To inhibit the environment which is favorable for bacterial growth, appropriate oral hygiene method and instruments should be used. Powered toothbrush, mouthwashes, and floss have been shown to improve control in the orthodontic patient. Choi et al. did a study to evaluate changes that occur in the subgingival microbiota after removal of fixed orthodontic appliances. They concluded that periodontopathogens during orthodontic treatment were significantly reduced within 3 months of appliance removal. A reduction of all bacterial counts was detected after the 3-month follow-up in all groups. Lower counts of P. gingivalis were achieved from 1 week after treatments. The 2% chlorhexidine concentration seemed to contribute to lower A. actinomycetemcomitans levels and increase Streptococcus mutans levels.
| Conclusion|| |
- Maximum increase in number was seen for P. intermedia and least for P. gingivalis
- For different microbes, change in microbial count ranged from 18.8% (P. gingivalis) to 52% (P. intermedia). For Veillonella, the change was 51.4%
- For anaerobes, the change was higher both for mean count as well as proportional change before and during treatment.
It can be concluded that orthodontic band placement can influence the aggregation and content of subgingival microbes, resulting in inflammation, bleeding, and periodontal damage. Hence, periodontal disease occurring during active orthodontic treatment should be prevented by giving patient special oral hygiene care.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
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[Figure 1], [Figure 2], [Figure 3]
[Table 1], [Table 2], [Table 3], [Table 4]